{"id":1231,"date":"2023-09-15T15:47:22","date_gmt":"2023-09-15T15:47:22","guid":{"rendered":"https:\/\/vishalfoodtech.com\/?p=1231"},"modified":"2023-09-15T15:47:23","modified_gmt":"2023-09-15T15:47:23","slug":"carbohydrate-determination","status":"publish","type":"post","link":"https:\/\/vishalfoodtech.com\/index.php\/2023\/09\/15\/carbohydrate-determination\/","title":{"rendered":"Determination of Carbohydrate by Phenol-Sulfuric Acid Method- Best Top 10 steps"},"content":{"rendered":"\n<p>Carbohydrate analysis is a crucial aspect of studying and understanding the composition and structure of various substances. One method that stands out is the determination of <strong><span style=\"text-decoration: underline;\">Carbohydrate by Phenol-Sulfuric Acid Method.<\/span><\/strong> This method offers a reliable and widely used approach for quantifying carbohydrates present in a sample. By harnessing the power of chemical reactions, the phenol-sulfuric acid method enables researchers to accurately determine the carbohydrate content and explore their implications in various fields such as food science, biochemistry, and pharmaceutical research. In this blog, we will delve into the intricacies of carbohydrate analysis by exploring the phenol-sulfuric acid method, its principles, applications, and best practices.<\/p>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_25 counter-hierarchy counter-decimal ez-toc-grey\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" style=\"display: none;\"><label for=\"item\" aria-label=\"Table of Content\"><i class=\"ez-toc-glyphicon ez-toc-icon-toggle\"><\/i><\/label><input type=\"checkbox\" id=\"item\"><\/a><\/span><\/div>\n<nav><ul class=\"ez-toc-list ez-toc-list-level-1\"><li class=\"ez-toc-page-1 ez-toc-heading-level-2\"><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2023\/09\/15\/carbohydrate-determination\/#Do_you_know_the_Principle\" title=\"Do you know the Principle ?\">Do you know the Principle ?<\/a><ul class=\"ez-toc-list-level-3\"><li class=\"ez-toc-heading-level-3\"><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2023\/09\/15\/carbohydrate-determination\/#Key_steps\" title=\"Key steps:\">Key steps:<\/a><\/li><li class=\"ez-toc-page-1 ez-toc-heading-level-3\"><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2023\/09\/15\/carbohydrate-determination\/#FOR_GROUND_POWDERED_SAMPLE\" title=\"FOR GROUND POWDERED SAMPLE:\">FOR GROUND POWDERED SAMPLE:<\/a><ul class=\"ez-toc-list-level-4\"><li class=\"ez-toc-heading-level-4\"><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2023\/09\/15\/carbohydrate-determination\/#Sample_preparation_for_Determination_of_Carbohydrate_by_Phenol-Sulfuric_Acid_Method\" title=\"Sample preparation for Determination of Carbohydrate by Phenol-Sulfuric Acid Method\">Sample preparation for Determination of Carbohydrate by Phenol-Sulfuric Acid Method<\/a><\/li><\/ul><\/li><\/ul><\/li><li class=\"ez-toc-page-1 ez-toc-heading-level-2\"><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2023\/09\/15\/carbohydrate-determination\/#Preparation_of_25_N_Hydrochloric_acid_HCI_solution\" title=\"Preparation of 2.5 N Hydrochloric acid (HCI) solution\">Preparation of 2.5 N Hydrochloric acid (HCI) solution<\/a><ul class=\"ez-toc-list-level-3\"><li class=\"ez-toc-heading-level-3\"><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2023\/09\/15\/carbohydrate-determination\/#Standard_Curve_Generation_for_the_Determination_of_Carbohydrate_by_Phenol-Sulfuric_Acid_Method\" title=\"Standard Curve Generation for the Determination of Carbohydrate by Phenol-Sulfuric Acid Method\">Standard Curve Generation for the Determination of Carbohydrate by Phenol-Sulfuric Acid Method<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Do_you_know_the_Principle\"><\/span><strong>Do you know the Principle ?<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>The principle lies in the reaction between carbohydrates and concentrated sulfuric acid in the presence of phenol. This reaction results in the formation of furfural compounds from the carbohydrates, and these furfural compounds further react with phenol to create a colored complex.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Key_steps\"><\/span><strong>Key steps:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>Initial Reaction<\/strong>: When concentrated sulfuric acid is added to a carbohydrate-containing sample, it dehydrates the carbohydrates, breaking them down into simpler compounds, primarily furfural and other intermediates. This step is critical as it converts carbohydrates into compounds that can react further.<\/li>\n\n\n\n<li><strong>Formation of Furfural Compounds<\/strong>: Furfural compounds are produced as a result of the dehydration reaction. These compounds are key intermediates in the subsequent color-forming reaction with phenol.<\/li>\n\n\n\n<li><strong>Reaction with Phenol<\/strong>: Phenol is added to the reaction mixture. Furfural compounds react with phenol to create a complex mixture of compounds, including colored products.<\/li>\n\n\n\n<li><strong>Color Development<\/strong>: The complex mixture formed in the presence of phenol includes compounds that absorb light in the visible spectrum. This absorption results in the development of a color directly proportional to the concentration of carbohydrates in the sample.<\/li>\n\n\n\n<li><strong>Spectrophotometric Analysis<\/strong>: The colored solution is then subjected to spectrophotometric analysis. A spectrophotometer measures the absorbance of light by the solution at a specific wavelength, usually between 480-490 nm. The intensity of this absorbance is directly related to the concentration of carbohydrates in the sample.<\/li>\n<\/ol>\n\n\n\n<p>By comparing the absorbance of the sample to a <a href=\"https:\/\/vishalfoodtech.com\/index.php\/2023\/09\/06\/gallic-acid-standard-prep\/\" data-type=\"post\" data-id=\"5555\">standard curve<\/a> created with known concentrations of a standard carbohydrate solution, the concentration of carbohydrates in the sample can be accurately determined.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"FOR_GROUND_POWDERED_SAMPLE\"><\/span>FOR GROUND POWDERED SAMPLE:<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<h4 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Sample_preparation_for_Determination_of_Carbohydrate_by_Phenol-Sulfuric_Acid_Method\"><\/span><strong>Sample preparation for Determination of Carbohydrate by Phenol-Sulfuric Acid Method<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h4>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1280\" height=\"591\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-3-edited.png\" alt=\"\" class=\"wp-image-1236\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-3-edited.png 1280w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-3-edited-300x139.png 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-3-edited-1024x473.png 1024w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-3-edited-768x355.png 768w\" sizes=\"auto, (max-width: 1280px) 100vw, 1280px\" \/><figcaption class=\"wp-element-caption\"><strong>These are the seven samples whose carbohydrate content is to be determined. A, A1, B, B1, C, D, E<\/strong><\/figcaption><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>0.1 g<\/strong> of ground samples were taken in test tubes; <\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"473\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-5-1024x473.png\" alt=\"\" class=\"wp-image-1238\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-5-1024x473.png 1024w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-5-300x139.png 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-5-768x355.png 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-5.png 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><strong>0.1g samples put in 7 test tubes each<\/strong><\/figcaption><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The samples were hydrolyzed by using <strong>5 ml<\/strong> of <strong>2.5 N Hydrochloric acid (HCI)<\/strong> solution in the water bath at temperature of <strong>100 \u00b0C \u00b1 1 \u00b0C<\/strong> for <strong>3 hrs<\/strong>.<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Preparation_of_25_N_Hydrochloric_acid_HCI_solution\"><\/span><strong><span style=\"text-decoration: underline;\">Preparation of 2.5 N Hydrochloric acid (HCI) solution<\/span><\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>Density= (Mass\/ Volume)<\/p>\n\n\n\n<p>We have to prepare <strong>2.5 N Hydrochloric acid (HCI)<\/strong> solution. <\/p>\n\n\n\n<pre class=\"wp-block-preformatted\">We know <strong>Normality=(Acidity or basicity)* Molarity<\/strong><\/pre>\n\n\n\n<p>HCL has an acidity of 1 because it can release only one proton (H+).<\/p>\n\n\n\n<p>So from the above formula, for HCL, Normality= Molarity (bez Acidity =1)<\/p>\n\n\n\n<p>Hence, 2.5N=2.5M<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"432\" height=\"124\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-8.png\" alt=\"\" class=\"wp-image-1241\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-8.png 432w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-8-300x86.png 300w\" sizes=\"auto, (max-width: 432px) 100vw, 432px\" \/><\/figure><\/div>\n\n\n<p>So, we can take the Volume of solution in litre=1 L and Molarity = 2.5M in the above formula, and no of moles comes out as 2.5 moles.<\/p>\n\n\n\n<p>now Density= (Mass\/ Volume)&#8212;&#8212;-<strong>1<\/strong><\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"928\" height=\"272\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-9.png\" alt=\"\" class=\"wp-image-1242\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-9.png 928w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-9-300x88.png 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-9-768x225.png 768w\" sizes=\"auto, (max-width: 928px) 100vw, 928px\" \/><\/figure><\/div>\n\n\n<p>Putting all these values, density of HCL= 1.18, Mol wt of HCL= 36.458, <\/p>\n\n\n\n<p>We can find Volume = (2.5*36.458)\/1.18 = 77.24ml per 1 Litre H2O.<\/p>\n\n\n\n<p>Since HCL was of 35% purity, divide by 0.35 &gt;&gt; (77.24\/0.35)= 220ml\/ 1000mL H2O. ( So, to make 2.5N HCL, take 220mL in 1000mL DW- distilled water, or if we want to make a smaller amount, we can take 22mL in 100mL DW, or even 2.2mL in 10mL DW)<\/p>\n\n\n\n<p>So now added 5mL of that prepared 2.5N HCL solution in the test tubes. (See figure below)<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/CamScanner-06-24-2023-12.21-Copy_1-473x1024.jpg\" alt=\"\" class=\"wp-image-1246\" width=\"225\" height=\"488\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/CamScanner-06-24-2023-12.21-Copy_1-473x1024.jpg 473w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/CamScanner-06-24-2023-12.21-Copy_1-139x300.jpg 139w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/CamScanner-06-24-2023-12.21-Copy_1-768x1663.jpg 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/CamScanner-06-24-2023-12.21-Copy_1-710x1536.jpg 710w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/CamScanner-06-24-2023-12.21-Copy_1-946x2048.jpg 946w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/CamScanner-06-24-2023-12.21-Copy_1.jpg 1068w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/figure><\/div>\n\n\n<p><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>All these centrifuge tubes were transferred to testtubes and were kept in the water bath at 100 degrees Celsius for 3 hours.<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-12-1024x768.png\" alt=\"Determination of Carbohydrate by Phenol-Sulfuric Acid Method phenol h2so4 method\" class=\"wp-image-5623\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-12-1024x768.png 1024w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-12-300x225.png 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-12-768x576.png 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-12.png 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"472\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-13-1024x472.png\" alt=\"Determination of Carbohydrate by Phenol-Sulfuric Acid Method phenol h2so4 method\" class=\"wp-image-1250\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-13-1024x472.png 1024w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-13-300x138.png 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-13-768x354.png 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-13-1536x708.png 1536w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/06\/image-13.png 1632w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The test tubes were plugged by using non-adsorbent cotton. After hydrolysis, the samples were allowed to cool down at ambient room temperature.<\/li>\n<\/ul>\n\n\n\n<p>After 3 hours, color changed to deep iron red as shown below in figure:<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-13-1024x768.png\" alt=\"Determination of Carbohydrate by Phenol-Sulfuric Acid Method\" class=\"wp-image-5624\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-13-1024x768.png 1024w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-13-300x225.png 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-13-768x576.png 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-13.png 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Sodium carbonate in solid form was added to the test tubes, until the effervescence ceases. Finally, the volume was made up to <strong>100 mL <\/strong>by adding distilled water<strong>.<\/strong><\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-14-768x1024.png\" alt=\"Determination of Carbohydrate by Phenol-Sulfuric Acid Method na2co3 effervescence\" class=\"wp-image-5625\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-14-768x1024.png 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-14-225x300.png 225w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-14.png 960w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-15-1024x768.png\" alt=\"Determination of Carbohydrate by Phenol-Sulfuric Acid Method\" class=\"wp-image-5626\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-15-1024x768.png 1024w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-15-300x225.png 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-15-768x576.png 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-15.png 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">Deep brown colour after adding Na2Co3 in the test tubes until the effervescence ceased<\/figcaption><\/figure><\/div>\n\n\n<ul class=\"wp-block-list\">\n<li>Now take 0.5ml from each and make up with 9.5ml Distilled water to dilute 100 times in another test tubes.<\/li>\n\n\n\n<li>The samples were centrifuged at 3000\u00d7g for 15 minutes. The supernatant was collected for the carbohydrate estimation.<\/li>\n\n\n\n<li>Collect the supernatant for the<a href=\"https:\/\/www.google.com\/url?sa=t&amp;rct=j&amp;q=&amp;esrc=s&amp;source=web&amp;cd=&amp;cad=rja&amp;uact=8&amp;ved=2ahUKEwjzwtyN9qyBAxVhT2wGHftoCsU4KBAWegQICRAB&amp;url=http%3A%2F%2Fwww.sciepub.com%2Freference%2F181155&amp;usg=AOvVaw07XTIb2MDyVIyQCg-rt-CB&amp;opi=89978449\" target=\"_blank\" rel=\"noreferrer noopener\"> carbohydrate estimation<\/a>. This supernatant will be further used.<\/li>\n\n\n\n<li>Below is the detailed step-by-step procedure till Supernatant collection.<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1592\" height=\"2560\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-17-scaled.jpg\" alt=\"Determination of Carbohydrate by Phenol-Sulfuric Acid Method\" class=\"wp-image-5637\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-17-scaled.jpg 1592w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-17-187x300.jpg 187w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-17-637x1024.jpg 637w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-17-768x1235.jpg 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-17-955x1536.jpg 955w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-17-1274x2048.jpg 1274w\" sizes=\"auto, (max-width: 1592px) 100vw, 1592px\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Standard_Curve_Generation_for_the_Determination_of_Carbohydrate_by_Phenol-Sulfuric_Acid_Method\"><\/span><strong>Standard Curve Generation<\/strong> for the Determination of Carbohydrate by Phenol-Sulfuric Acid Method<span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-16-1024x768.png\" alt=\"\" class=\"wp-image-5640\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-16-1024x768.png 1024w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-16-300x225.png 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-16-768x576.png 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-16.png 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">As shown above, the standards prepared of conc 10, 20, 40, 60, 80 and 100 microgram\/litre. The colour darkens and intensity increases as the concentration is increased from 10 to 100 micrograms\/litre.<\/figcaption><\/figure><\/div>\n\n\n<p>Now the below-shown figure is of samples after the Concentrated sulphuric acid addition.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-17-1024x768.png\" alt=\"\" class=\"wp-image-5645\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-17-1024x768.png 1024w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-17-300x225.png 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-17-768x576.png 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/image-17.png 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p> You can now plot the standard curve and get the value of x which is concentration of carbohydrates present in your sample.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-18-1024x643.jpg\" alt=\"Standard Curve Generation for the Determination of Carbohydrate by Phenol-Sulfuric Acid Method\" class=\"wp-image-5646\" width=\"820\" height=\"514\" srcset=\"https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-18-1024x643.jpg 1024w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-18-300x188.jpg 300w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-18-768x482.jpg 768w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-18-1536x965.jpg 1536w, https:\/\/vishalfoodtech.com\/wp-content\/uploads\/2023\/09\/Page-18-2048x1286.jpg 2048w\" sizes=\"auto, (max-width: 820px) 100vw, 820px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Carbohydrate analysis is a crucial aspect of studying and understanding the composition and structure of various substances. One method that stands out is the determination of Carbohydrate by Phenol-Sulfuric Acid Method. This method offers a reliable and widely used approach for quantifying carbohydrates present in a sample. By harnessing the power of chemical reactions, the&hellip;&nbsp;<a href=\"https:\/\/vishalfoodtech.com\/index.php\/2023\/09\/15\/carbohydrate-determination\/\" rel=\"bookmark\">Read More &raquo;<span class=\"screen-reader-text\">Determination of Carbohydrate by Phenol-Sulfuric Acid Method- Best Top 10 steps<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":5650,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"","neve_meta_content_width":0,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","_themeisle_gutenberg_block_has_review":false,"footnotes":""},"categories":[92],"tags":[17],"class_list":["post-1231","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-food-technology-analytical-techniques","tag-food-tech"],"_links":{"self":[{"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/posts\/1231","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/comments?post=1231"}],"version-history":[{"count":22,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/posts\/1231\/revisions"}],"predecessor-version":[{"id":5649,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/posts\/1231\/revisions\/5649"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/media\/5650"}],"wp:attachment":[{"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/media?parent=1231"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/categories?post=1231"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/tags?post=1231"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}