{"id":6444,"date":"2026-07-25T00:56:25","date_gmt":"2026-07-25T00:56:25","guid":{"rendered":"https:\/\/vishalfoodtech.com\/?p=6444"},"modified":"2026-07-25T00:58:37","modified_gmt":"2026-07-25T00:58:37","slug":"ftir-protein-secondary-structure-originpro","status":"publish","type":"post","link":"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/","title":{"rendered":"FTIR Protein Secondary Structure Analysis Using OriginPro (12-step Complete Amide I Deconvolution Tutorial)"},"content":{"rendered":"\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_86 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#FTIR_Protein_Secondary_Structure_Analysis_Using_OriginPro\" >FTIR Protein Secondary Structure Analysis Using OriginPro<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#Watch_the_Complete_Tutorial\" >Watch the Complete Tutorial<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#What_You_Will_Learn\" >What You Will Learn<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#Software_Used\" >Software Used<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#Equipment_Used\" >Equipment Used<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#Why_Analyse_the_Amide_I_Region\" >Why Analyse the Amide I Region?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#Peak_Assignments_Used\" >Peak Assignments Used<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#Secondary_Structure_Calculation\" >Secondary Structure Calculation<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#Free_Excel_Calculator\" >Free Excel Calculator<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#Who_Is_This_Tutorial_For\" >Who Is This Tutorial For?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#Related_Topics\" >Related Topics<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-12\" href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/#About_Vishal_Food_Tech\" >About Vishal Food Tech<\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"FTIR_Protein_Secondary_Structure_Analysis_Using_OriginPro\"><\/span>FTIR Protein Secondary Structure Analysis Using OriginPro<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Protein secondary structure analysis using Fourier Transform Infrared (FTIR) spectroscopy has become one of the most widely used analytical techniques in food science, protein chemistry, biotechnology and pharmaceutical research. Despite its widespread use, many researchers struggle with one important step\u2014converting an FTIR spectrum into meaningful percentages of \u03b2-sheet, \u03b1-helix, random coil and \u03b2-turn structures.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">This tutorial provides a complete workflow starting from sample analysis on a PerkinElmer FTIR instrument through to Gaussian peak deconvolution in OriginPro and final secondary structure calculations.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Watch_the_Complete_Tutorial\"><\/span>Watch the Complete Tutorial<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>(<a href=\"https:\/\/youtu.be\/zngdAz0SBhU\" target=\"_blank\" rel=\"noopener\">FTIR Protein Secondary Structure Analysis in OriginPro | Complete Amide I Deconvolution Tutorial<\/a>)<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<div class=\"nv-iframe-embed\"><iframe loading=\"lazy\" title=\"FTIR Protein Secondary Structure Analysis in OriginPro | Complete Amide I Deconvolution Tutorial\" width=\"1200\" height=\"675\" src=\"https:\/\/www.youtube.com\/embed\/zngdAz0SBhU?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/div>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"What_You_Will_Learn\"><\/span>What You Will Learn<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">In this tutorial you will learn:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>FTIR fundamentals<\/li>\n\n\n\n<li>Protein secondary structure<\/li>\n\n\n\n<li>ATR-FTIR sample loading<\/li>\n\n\n\n<li>Amide I region<\/li>\n\n\n\n<li>Why baseline correction is required<\/li>\n\n\n\n<li>Why the Amide I region is analysed<\/li>\n\n\n\n<li>Gaussian peak fitting<\/li>\n\n\n\n<li>Choosing peak centres<\/li>\n\n\n\n<li>Fixing peak centres<\/li>\n\n\n\n<li>Setting peak width bounds<\/li>\n\n\n\n<li>Area calculations<\/li>\n\n\n\n<li>Calculating secondary structure percentages<\/li>\n\n\n\n<li>Exporting publication-quality figures<\/li>\n\n\n\n<li>Preparing results for research papers<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Software_Used\"><\/span>Software Used<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>PerkinElmer Spectrum IR<\/li>\n\n\n\n<li>OriginPro<\/li>\n\n\n\n<li>Microsoft Excel<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Equipment_Used\"><\/span>Equipment Used<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>PerkinElmer Spectrum Two FTIR<\/li>\n\n\n\n<li>Universal ATR crystal<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Why_Analyse_the_Amide_I_Region\"><\/span>Why Analyse the Amide I Region?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">The Amide I band (1600\u20131700 cm\u207b\u00b9) originates mainly from the C=O stretching vibration of peptide bonds.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Because this vibration is highly sensitive to hydrogen bonding and molecular conformation, the Amide I region provides valuable information about protein secondary structure.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Peak_Assignments_Used\"><\/span>Peak Assignments Used<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><thead><tr><th>Peak<\/th><th>Assignment<\/th><\/tr><\/thead><tbody><tr><td>1620<\/td><td>\u03b2-sheet<\/td><\/tr><tr><td>1632<\/td><td>\u03b2-sheet<\/td><\/tr><tr><td>1642<\/td><td>Random coil<\/td><\/tr><tr><td>1654<\/td><td>\u03b1-helix<\/td><\/tr><tr><td>1670<\/td><td>\u03b2-turn<\/td><\/tr><tr><td>1688<\/td><td>High-frequency \u03b2-sheet<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Secondary_Structure_Calculation\"><\/span>Secondary Structure Calculation<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">The tutorial demonstrates calculation of:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u03b2-sheet (%) = ((P1 + P2 + P6) \/ Total Area) \u00d7 100<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Random coil (%) = (P3 \/ Total Area) \u00d7 100<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u03b1-helix (%) = (P4 \/ Total Area) \u00d7 100<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u03b2-turn (%) = (P5 \/ Total Area) \u00d7 100<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Free_Excel_Calculator\"><\/span>Free Excel Calculator<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">The Excel workbook used in this tutorial is freely available.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Download it here:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">(<a href=\"https:\/\/docs.google.com\/spreadsheets\/d\/1JxMIu665hYVV-kLSYH8SY80KQc-uwmZX\/edit?usp=sharing\" target=\"_blank\" rel=\"noopener\">Free Excel Calculator For Secondary Structure Calculation<\/a>)<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Who_Is_This_Tutorial_For\"><\/span>Who Is This Tutorial For?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">This tutorial is useful for:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Undergraduate students<\/li>\n\n\n\n<li>MSc students<\/li>\n\n\n\n<li>PhD researchers<\/li>\n\n\n\n<li>Food scientists<\/li>\n\n\n\n<li>Protein chemists<\/li>\n\n\n\n<li>Biotechnologists<\/li>\n\n\n\n<li>Pharmaceutical researchers<\/li>\n\n\n\n<li>Analytical chemists<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Related_Topics\"><\/span>Related Topics<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>FTIR Spectroscopy<\/li>\n\n\n\n<li>ATR-FTIR<\/li>\n\n\n\n<li><a href=\"https:\/\/vishalfoodtech.com\/index.php\/tag\/classification-and-structure-of-protein-in-food\/\" data-type=\"post_tag\" data-id=\"97\">Protein<\/a> Structure<\/li>\n\n\n\n<li>Gaussian Curve Fitting<\/li>\n\n\n\n<li>OriginPro Tutorials<\/li>\n\n\n\n<li>Spectral Deconvolution<\/li>\n\n\n\n<li><a href=\"https:\/\/vishalfoodtech.com\/index.php\/category\/gate-food-technology\/\" data-type=\"category\" data-id=\"88\">Food<\/a> Protein Analysis<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"About_Vishal_Food_Tech\"><\/span>About Vishal Food Tech<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Vishal Food Tech provides tutorials focused on food science research, analytical techniques, spectroscopy, scientific software and publication-ready data analysis.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">New tutorials are published regularly.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<div class=\"nv-iframe-embed\"><iframe loading=\"lazy\" title=\"FTIR Protein Secondary Structure Analysis in OriginPro | Complete Amide I Deconvolution Tutorial\" width=\"1200\" height=\"675\" src=\"https:\/\/www.youtube.com\/embed\/zngdAz0SBhU?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/div>\n<\/div><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<figure class=\"wp-block-embed\"><div class=\"wp-block-embed__wrapper\">\nhttps:\/\/shop.perkinelmer.com\/en-IE\/product\/L160000A\n<\/div><\/figure>\n\n\n\n<figure class=\"wp-block-embed\"><div class=\"wp-block-embed__wrapper\">\nhttps:\/\/www.originlab.com\n<\/div><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>FTIR Protein Secondary Structure Analysis Using OriginPro Protein secondary structure analysis using Fourier Transform Infrared (FTIR) spectroscopy has become one of the most widely used analytical techniques in food science, protein chemistry, biotechnology and pharmaceutical research. Despite its widespread use, many researchers struggle with one important step\u2014converting an FTIR spectrum into meaningful percentages of \u03b2-sheet,&hellip;&nbsp;<a href=\"https:\/\/vishalfoodtech.com\/index.php\/2026\/07\/25\/ftir-protein-secondary-structure-originpro\/\" rel=\"bookmark\">Read More &raquo;<span class=\"screen-reader-text\">FTIR Protein Secondary Structure Analysis Using OriginPro (12-step Complete Amide I Deconvolution Tutorial)<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":6446,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"","neve_meta_content_width":0,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","_themeisle_gutenberg_block_has_review":false,"footnotes":""},"categories":[8,92],"tags":[],"class_list":["post-6444","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","category-food-technology-analytical-techniques"],"_links":{"self":[{"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/posts\/6444","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/comments?post=6444"}],"version-history":[{"count":3,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/posts\/6444\/revisions"}],"predecessor-version":[{"id":6452,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/posts\/6444\/revisions\/6452"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/media\/6446"}],"wp:attachment":[{"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/media?parent=6444"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/categories?post=6444"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/vishalfoodtech.com\/index.php\/wp-json\/wp\/v2\/tags?post=6444"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}